Figure 2. Illustration of PTGS experiments using agroinfiltration and micrografting.

(a) A four-week-old plant was stripped of all the lateral leaves except for L7 to encourage the establishment of a source L7 leaf and a sink for newly developing leaves. Seven days later, the leaves that emerged (marked by square) from the apex were sampled. (b) Five-day-old seedlings were grafted using a silicone tube. After 7 days of culture on MS agar, the tube was removed, and then the graft plant was transferred to rockwool soaked with nutrient solution. After 10 days, all the leaves were cut off to encourage the establishment of a root source and emerging leaf sink, and then after another 7 days the newly developed leaves were observed and sampled.