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. 2011 Feb 9;6(2):e16870. doi: 10.1371/journal.pone.0016870

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Vandevenne et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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HEK-293 cells were transfected either with HA-tagged ORF47 or V5-tagged IRF3 or co-transfected with both plasmids. 24 hours later, cells were infected or not with the mutant VZV ROka47S for another 24 hours. Either IRF3 or HA-ORF47 was immunoprecipitated from total extracts with anti-IRF3 and anti-HA antibodies. Inputs and immune complexes were then analyzed by Western Blotting using specific antibodies against HA-tag and IRF3. A negative control without antibody was also performed.