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. 2011 Feb 9;6(2):e17031. doi: 10.1371/journal.pone.0017031

Figure 6. Up-regulation of E-cadherin following NAT1 knock-down is not cell-type specific.

Figure 6

(A) 22Rv1 cells were stably transfected with the NAT1-directed shRNA construct (Clones 1 and 2) and knock-down determined by NAT1 activity assay. Results are presented as mean ± SEM (n = 3). Asterisks denote significant difference compared to the control (p<0.05). (B) Western blot analysis of E-cadherin protein in 22Rv1 control and NAT1 knock-down cells. Immunoblotting was performed with E-cadherin antibody and tubulin was probed as the loading control. The blot is representative of 3 independent experiments. Immunoblots were quantified by densitometry after normalization to tubulin. Results are presented as means ± SEM (n = 3). A representative blot is shown above the graph. Asterisks denote significant difference compared to control (P<0.05). (C) Immunocytochemistry of E-cadherin expression in 22Rv1 control and NAT1 knock-down cells. E-cadherin was detected with anti-E-cadherin antibody followed by Alexa Fluor 488-conjugated secondary antibody (green) and visualized under a fluorescence microscope. The nuclei were stained with DAPI (blue).