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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Leukemia. 2010 Nov 12;25(2):305–312. doi: 10.1038/leu.2010.257

Figure 4. Effect of Grb2 knock down on CD34+ cell apoptosis and differentiation.

Figure 4

Transduced CD34+ cells were cultured with low GF for indicated periods and analyzed by flow cytometry for apoptosis and erythroid and myeloid markers. (A) Cells were labeled with Annexin-V Cy-5 and DAPI and apoptosis assessed by flow cytometry. Significance values: * P < 0.015, MIG 210+ctr shRNA versus Grb2-2 shRNA; ** P < 0.006, MIG 210+ctr shRNA versus Grb2-5 shRNA. (B) Cells were analyzed for glycophorin A (GlyA), CD 11b, CD 14 and CD 33 expression on day 7 and 14. The figure shows absolute number (log10) of cells per 100,000 input cells. Significance values for differences between MIG 210 + ctr shRNA and various Grb2 shRNAs are indicated * P < 0.05.