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Schematic representation of recombinase-mediated selectable marker removal. The marker gene is flanked by directly oriented recombinase recognition sites (red arrows). The excision event removes the DNA between the associated recognition sites leaving the external DNA, such as the gene of interest (GOI) intact and a single recognition site behind in the genome. The non-replicating circular DNA fragment is lost. The recombinase can be provided in cis or in trans—not shown
