Fig. 3.

Schematic representation of an inducible recombinase-mediated selectable marker removal strategy. The marker and recombinase genes are flanked by directly oriented recombinase recognition sites (red arrows). In this strategy the recombinase gene is present in the genome and can be externally or developmentally induced. Activation of the recombinase causes excision of the recombinase and marker gene, leaving behind only the gene of interest (GOI), a single recognition site and a non-replicating circular DNA fragment