Fig. 4.

Minigene Functional Splicing Assay. a The hybrid minigene (pCalfaM) used in transient transfection splicing assays in HeLa cells is shown. Exons are indicated as boxes. Introns are indicated as lines. MLH1 Exon 9 and 10 (white and gray box) were tested for splicing efficiency by using specific primers (arrows). b RT-PCR products from transfection experiments. RNA splicing variants corresponding to wt (2) exon 9 exclusion (3) and both exon 9 and 10 exclusion (4) are shown. (1) indicate a splice variant generated as an artefact of the minigene. M is the 100 bp marker