Figure 2. Rapamycin altered IFN-γ production in antigen-specific T cell populations following pathogen infection.

A, Splenocytes from LM-OVA infected mice, which were either left untreated or treated with rapamycin were restimulated with OVA peptide and stained for the presence of IFN-γ or TNF. B, Treatment with rapamycin resulted in an increase in the number of total IFN-γ⁺ T cells. C, Splenocytes from mOVA grafted mice, which were untreated or treated with rapamycin, were restimulated with OVA peptide and stained for the presence of IFN-γ or TNF. D, Treatment with rapamycin did not result in an increase in the number of total IFN-γ⁺ T cells. Experiments were performed three times independently with 5 mice per group.