Figure 1.
Schematic diagram of B. subtilis NCD-2 mdh, phoP, phoR and polA genes. Single-headed arrows represent the location and orientation of genes in the B. subtilis NCD-2 chromosome. Main restriction enzyme sites are shown, the mini-Tn10 in M216 mutants being inserted into the phoR gene. For M216 mutant phoR gene complementation, the gene itself was inserted into a pHY300PLK plasmid.