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. 2010 Jun 1;33(2):333–340. doi: 10.1590/S1415-47572010005000032

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Copyright © 2010, Sociedade Brasileira de Genética.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Growth and alkaline phosphatase (APase) activities of B. subtilis NCD-2 and its derivative strains during growth in both a high and low phosphate medium. Samples were removed periodically, and APase activities assayed in cell extracts. The values are averages of three replications, with mean values ± SD indicated. Squares, NCD-2 wild-type; triangles, M216 mutant; circles, M216 mutant complemented strain. Open symbols, cell growth in a high phosphate medium; closed symbols, cell growth in a low phosphate medium.