Figure 3.
Effects of simple repeats constructed from small A-rich ORF2 fragments on GFP gene expression. AAACAAA or AG 736-bp long repeats were inserted in sense or antisense orientation downstream of GFP. The inserts inhibited GFP transcription. AAACAAA repeats in either orientation induced premature transcriptional termination. AG repeats in antisense orientation produced a greater number of higher molecular mass transcripts than in sense orientation. Arrows indicate positions of low or high molecular mass transcripts.