Table 1.

Primer pairs and optimized conditions used for determining bovine gene expression by quantitative real-time PCR

Gene	Oligonucleotides (5'-3') F: forward; R: reverse	Reference	Primer (nM)	cDNA (ng/μL)	DT (°C)
IL-2	F: GGATTTACAGTTGCTTTTGGAGAAA R: GCACTTCCTCTAGAAGTTTGAGTTCTT	1	400	400	65.4
IL-4	F: CATGCATGGAGCTGCCTGTA R: AATTCCAACCCTGCAGAAGGT	2	400	200	79.3
IL-6	F: TCAGCTTATTTTCTGCCAGTCTCT R: TCATTAAGCACATCGTCGACAAA	1	400	400	72.6
IL-8	F: CACTGTGAAAAATTCAGAAATCATTGTTA R: CTTCACCAAATACCTGCACAACCTTC	1	400	100	77.2
IL-10	F: CCAAGCCTTGTCGGAAATGA R: GTTCACGTGCTCCTTGATGTCA	3	200	100	80.2
TNF-α	F: TCTTCTCAAGCCTCAAGTAACAAGT R: CCATGAGGGCATTGGCATAC	1	400	100	83.0
IFN-γ	F: TGGATATCATCAAGCAAGACATGTT R: ACGTCATTCATCACTTTCATGAGTTC	1	400	200	87.5
GAPDH	F: GGCGTGAACCACGAGAAGTATAA R: CCCTCCACGATGCCAAAGT	1	400	100	82.6

References: ¹Leutenegger et al., 2000; ²Waldvogel et al. 2000; ³Moussay et al., 2006.