Table 2.
- Summary of results obtained with the Drosophila wing spot test (SMART) in the marker-trans-heterozygous (MH) and balancer-heterozygous (BH) progeny of the high bioactivation (HB) cross after chronic treatment of larvae with Tabebuiaimpetiginosa (Ti) and doxorubicin (DXR).
| Genotypes and treatment | Number of flies | Spot for fly (number of spots) statistical diagnosisa |
Spots with mwh cloneb (n) | Frequency of clone formation/105 cellsc |
Recombination (%) | Inductiond (%) | |||||
| DXR (mg mL-1) |
Ti (%) | Small single spots (1-2 cells)e | Large single spots (> 2 cells)e | Twin spots | Total spots | Observed | Control corrected | ||||
| MH3 | |||||||||||
| 0 | 0 | 40 | 0.85 (34) | 0.00 (00) | 0.01 (04) | 0.95 (38) | 38 | 1.95 | |||
| 0 | 10 | 38 | 0.97 (37) | 0.08 (03) | 0.13 (05) | 1.18 (45) | 45 | 2.43 | 0.48 | ||
| 0 | 20 | 40 | 0.85 (34) | 0.08 (03) | 0.00 (00) | 0.93 (37) | 37 | 1.90 | -0.05 | ||
| 0 | 40 | 32 | 0.63 (20) | 0.03 (01) | 0.06 (02) | 0.72 (23) | 23 | 1.47 | -0.47 | ||
| 0.125 | 0 | 40 | 2.10 (84)+ | 1.55 (62)+ | 2.58(103)+ | 6.23 (249)+ | 241 | 12.35 | 10.40 | 87.50 | |
| 0.125 | 10 | 40 | 3.35 (134)* | 3.20 (128)* | 4.65 (186)* | 11.20 (448)* | 433 | 22.17 | 20.24 | 87.00 | 94.61 |
| 0.125 | 20 | 38 | 3.45 (131)* | 3.26 (124)* | 3.84 (146)* | 10.55(401)* | 376 | 20.28 | 18.33 | 90.30 | 76.25 |
| 0.125 | 40 | 35 | 2.71 (95) | 1.77 (62) | 3.06 (107) | 7.54 (264)* | 256 | 14.99 | 12.87 | 90.00 | 23.75 |
| BH3 | |||||||||||
| 0 | 0 | 40 | 0.20 (08) | 0.00 (00) | f | 0.20 (08) | 8 | 0.41 | |||
| 0.125 | 0 | 41 | 0.68 (28)+ | 0.10 (04) | 0.78 (32)+ | 32 | 1.60 | 1.19 | |||
| 0.125 | 10 | 40 | 1.20 (48)* | 0.28 (11) | 1.48 (59)* | 59 | 3.02 | 2.61 | 119.33 | ||
| 0.125 | 20 | 40 | 1.00 (40) | 0.03 (01) | 1.03 (41) | 41 | 2.10 | 1.69 | |||
| 0.125 | 40 | 31 | 0.74 (23) | 0.03 (01) | 0.77 (24) | 24 | 1.59 | 1.06 | |||
MH flies (mwh/flr3) and BH flies (mwh/TM3) were evaluated. aStatistical diagnoses according to Frei and Würgler (1995). U-test, two-sided, probability levels: +, p ≤ 0.05 vs. untreated control; *, p ≤ 0.05 vs. DXR only. bConsidering mwh clones from mwh single and twin spots. cFrequency of clone formation: clones/flies/48,800 cells (without size correction). dCalculated as [DXR alone - (DXR + Ti) / DXR alone] X 100, according to Abraham (1994). eIncluding rare flr3 single spots. fOnly mwh single spot can be observed in BH individuals.