Figure 2.
A selective A2AAR agonist, ATL313, triggers cAMP accumulation in helper T cells. Purified human CD4+ Th cells were activated for 2h and treated for 10 min with 1 U ml −1 ADA in the presence of vehicle or 1 µM rolipram±1,000 nm ATL313 or forskolin, a positive control for cAMP accumulation. Intracellular cAMP accumulation was measured using a chemiluminescent immunoassay system. Data shown are the mean±s.e.m. from three independent experiments performed in triplicate. * Significant difference (P < 0.05) between the ATL313-treated and ATL313-untreated cells.