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. 2010 Dec 15;50(4):581–590. doi: 10.1021/bi101192f

Table 2. Kinetic Parameters for Primer Extension for Mismatched DNA Duplexes.

P/Ta kpol (s−1) Kd,app (μM) kpol/Kd,app (s−1 μM−1)
P/T1 169 70 2.4
P/T2b ND ND 4.8 × 10−4
P/T3b ND ND 1.3 × 10−3
P/T4b ND ND 1.5 × 10−2
P/T5 85 77 1.1
P/T6 163 62 2.6
a

Primer/Template (P/T) sequences are shown below:aThe mismatched base pairs are highlighted in red.

b

Because of nonsaturating conditions for incoming dNTPs, only the incorporation efficiency of the kcat/KM ratios was determined. Individual kpol and Kd,app values cannot be determined (ND).