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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Biochim Biophys Acta. 2010 Dec 22;1811(3):163–169. doi: 10.1016/j.bbalip.2010.12.006

Figure 1.

Figure 1

Recombinant rat Acsl3, Acsl4, Acsl6v1 and Acsl6v2-Flag proteins expressed in E. Coli were analyzed by Western blotting with an anti-Flag M2 monoclonal antibody. Protein-antibody complexes were visualized by chemiluminescence detection of horseradish peroxidase linked to goat anti-mouse IgG. The molecular mass of the recombinant Acsl isoenzymes was 74-kDa. The same E. Coli strain containing the identical plasmid without the gene encoding for Acsl was used as control.