Table 1. In Vitro Purified COX-1 and COX-2 and Lipopolysaccaride (LPS)-Activated Macrophage-Like (RAW254.7 Cell) Cellular COX-2 Enzyme Inhibition Assay Data of Isomeric Iodo Compounds 1−10.
| IC50(μM)a |
|||
|---|---|---|---|
| compd no. | purified COX-1 | purified COX-2 | RAW 264.7 cell COX-2 |
| 1 | >4 | 0.08 | 0.04 |
| 2 | >4 | 3.80 | NT |
| 3 | >4 | 0.56 | NT |
| 4 | >4 | 0.26 | 2.70 |
| 5 | >4 | >4 | NT |
| 6 | >4 | >4 | NT |
| 7 | >4 | 0.32 | NT |
| 8 | >4 | 0.05 | 0.03 |
| 9 | >4 | >4 | NT |
| 10 | >4 | 3.00 | NT |
| celecoxib | >4 | 0.03 | NT |
a
IC50 values were determined by incubating several concentrations of inhibitors in DMSO with purified murine COX-2 (66 nM) and ovine COX-1 (44 nM) for 20 min followed by treatment with 1-14C-AA (50 mM) at 37 °C for 30 s. Assays were run in duplicate. NT, not tested.