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. 2011 Jan 19;8(1):161–184. doi: 10.3390/ijerph8010161

Figure 1.

Figure 1

Figure 1

LO synthesis, processing and molecular composition. A, LO synthesis and processing. LO synthesis and processing were determined by [35S]-methionine labeling and cycloheximide chase (5 h) assays. 1 and 3, 0 time chase; 2 and 4, 5 h chase; 1 and 2, cell extracts; 3 and 4, conditioned media. Note that at 0 time chase the cell extract contains the 46 kDa prepro-LO, the 50 kDa pro-LO, and the membrane-bound 32 kDa mature LO while the conditioned medium contains the secreted 50 kDa pro-LO and the processed 32 kDa mature-LO; at 5 h chase all LO species in the cell extract were processed and secreted into the extracellular space, and further cleaved to the 32 kD mature enzyme. B, amino acid sequences of rat and human LO. SPCS, the signal peptide cleavage site; GS, the N-glycosylation site; PPCS, the propeptide cleavage site; AS, the active site; CuBS, the copper-binding site; asterisks indicate the different amino acid composition of LO between human and rat.