Table 1.
Oligonucleotide primers used for real-time RT-PCR analysis.
| Gene | DNA Sequences (5′–3′) | PCR Product Size |
|---|---|---|
| Antioxidant Enzyme Proteins (Peroxiredoxins) | ||
| Prx1 | Forward Primer TGGCTCGACCCTGCTGATAG | 61 |
| Reverse Primer GGAGCAGGATACCCAATTTTTG | ||
| Prx2 | Forward Primer CCCCTGAATATCCCTCTGCTT | 57 |
| Reverse Primer CGCCGTAATTCTGGGACAA | ||
| Prx3 | Forward Primer GGCCCCATTTCTTGGAT | 60 |
| Reverse Primer CAGGGCAGGCTAAGGGAAAG | ||
| Prx4 | Forward Primer CCTGTTGCGGACCGAATCT | 55 |
| Reverse Primer GGGTCCGGAACCGTTCAT | ||
| Prx5 | Forward Primer CCCGATCAAGGTGGGAGAT | 56 |
| Reverse Primer CCCGGTTCCCCTTCAAATA | ||
| Prx6 | Forward Primer TCTGGCAAAAAATACCTCCGTTA | 58 |
| Reverse Primer GCCCCAATTTCCGCAAAG | ||
| Neuroprotective Genes | ||
| FOXOs1 | Forward Primer CCCGTCCTAGGCACGAACT | 69 |
| Reverse Primer ACGCGCCCAGAACTTAACTTC | ||
| PGC1α | Forward Primer GGACAGTCTCCCCGTGGAT | 57 |
| Reverse Primer TCCATCTGTCAGTGCATCAAATG | ||
| NMDAR | Forward Primer GGTCAGTTCTGTCCTGCACATC | 65 |
| Reverse Primer TGACTCTCCCGCGGAAAC | ||
| Mitochondrial Electron Transport Chain Genes | ||
| Complex I-NADHsubunit1 | Forward Primer CGGGCCCCCTTCGAC | 72 |
| Reverse Primer GGCCGGCTGCGTATTCT | ||
| Complex II Cyt. B | Forward Primer TTATCGCGGCCCTAGCAA | 70 |
| Reverse Primer TAATCCTGTTGGGTTGTTTGATCC | ||
| Complex IV COX1 | Forward Primer GAAGAGACAGTGTTTCATGTGGTGT | 75 |
| Reverse Primer TCCTGGGCCTTTCAGGAATA | ||
| Complex V ATPase-6 | Forward Primer TGTGGAAGGAAGTGGGCAA | 73 |
| Reverse Primer CCACTATGAGCTGGAGCCGT | ||
| Housekeeping Genes | ||
| Beta Actin | Forward Primer ACGGCCAGGTCATCACTATTC | 65 |
| Reverse Primer AGGAAGGCTGGAAAAGAGCC | ||
| GAPDH | Forward Primer TTCCCGTTCAGCTCTGGG | 59 |
| Reverse Primer CCCTGCATCCACTGGTGC | ||