Fig. 6.

Glucosensing is attenuated in GnRH neurons from metformin (met)-treated compared with untreated mice. A, Representative graphs of firing rate over time. Shaded region indicates period of low (0.2 mm) glucose application. Double-headed arrows in first graph indicate periods during which frequency was averaged for analysis. B, Firing rates in 5 mm glucose and during time intervals t1 and t2 after low-glucose application. Firing rate was significantly reduced by low glucose in GnRH neurons from untreated CON and PNA mice but not those treated with metformin (CON, n = 8 cells from five mice; PNA, n = 8 cells from four mice; CON metformin, n = 6 cells from three mice; and PNA metformin, n = 8 cells from three mice). *, P < 0.05 vs. 5 mm.