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. 2010 Dec 29;152(2):483–494. doi: 10.1210/en.2010-0774

Fig. 3.

Fig. 3.

RSV infection repression of dexamethasone induction of endogenous GR-regulated genes and proteins in A549 cells. A549 cells were mock inoculated with HeLa-conditioned media (closed square and black bar) or inoculated with live rgRSV (open square and white bar) or UV-irradiated rgRSV (open circle with dashed line and hatched bar) (MOI = 3) for 1 h followed by treatment with dexamethasone for 5 h. Dexamethasone (Dex) induction of GILZ (A and B), FKBP51 (C and D), and MKP-1 (E and F) was determined by real-time PCR and normalized to GAPDH and CAP-1. Means and sd are shown (n = 4). G and H, A549 cells were either inoculated with HeLa-conditioned media (mock infected) (black bar) or rgRSV (MOI = 3) (white bar) for 1 h followed by incubation with increasing concentrations of dexamethasone for 24 h. Western blotting of 10 μg total protein was performed for FKBP51 and stripped and reprobed for β-actin. A representative Western blotting is shown (G) and quantification using the Kodak Image Station 4000MM Pro (H). Means and sd are shown (n = 3).