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Inhibition of mTOR does not increase p27Kip1 in insulin resistant VSMCs. Representative Western blottings (WB) and densitometric analysis measuring levels of p70S6kinase phosphorylation (A) and p27Kip1 protein levels (B) in lysates obtained from WT- and L2-VSMCs after serum stimulation for 1 h (p70S6kinase) or 24 h (p27Kip1) in the presence of the indicated doses of rapamycin (F = 14.49; †, P < 0.001, ANCOVA).
