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. Author manuscript; available in PMC: 2011 Feb 10.
Published in final edited form as: Mol Microbiol. 2007 Oct 5;66(4):948–960. doi: 10.1111/j.1365-2958.2007.05967.x

Table 3.

TraG coupling protein is required for pTJ5a mobilization.

Plasmid(s) in donor straina Description Transfer frequencyb RP4/input donor Transfer phenotype Mobilization frequencyc of pLV22a Mob phenotype
None Control N/A N/A < 1 × 10−8 −
pDB126 RP4 mpf+ and dtr+ (3.4 ± 0.3 × 10−1)d + (1.1 ± 0.2 × 10−4)d +
pDB127 RP4 mpf+ and dtr+:traG0 < 1 × 10−8 − < 1 × 10−8 −
pDB127, pBS140 RP4 mpf+ and dtr+:traG0 + TraG (0.9 ± 0.2 × 10−1)d + (3.0 ± 1.1 × 10−4)d +
a

All donors (HB101 Smr) contained plasmid pTJ5a Tcr (pLV22a cloned in pACYC184).

b

Transfer frequency is equal to the number of transferred plasmid (pDB126/pDB127) transconjugants per input donor (HB101 Smr). Transconjugants were selected for expression of Cm (from pDB126/pDB127) and Nx (from HB101 Nxr) resistance.

c

The mobilization frequency of pLV22a is determined in the same experiment which is used to determine RP4 transfer frequency.

d

Mean of three experiments ± standard error of the mean.

Note: N/A means not applicable.