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. Author manuscript; available in PMC: 2011 Feb 10.
Published in final edited form as: Proteomics Clin Appl. 2009 Aug;3(8):989–999. doi: 10.1002/prca.200900045

Figure 3.

Figure 3

Representative 2D gel images obtained using urine samples collected via (A) Spontaneous void (B) Gauze insert diapers (C) Non-gel diapers and (D) Non-gel diaper with fecal contamination. Total urine protein (75 µg) was loaded onto pre-cast 7 cm IPG strips (pH 3–10 range nonlinear) for isoelectric focusing. Proteins were then separated in the second dimension using 4–12% gradient Bis-Tris NuPAGE® pre-cast slab gels (7 cm). Protein spots were visualized by staining the gels with SYPRO Ruby.