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. 2011 Jan 30;4(2):124–133.

Figure 4.

Figure 4

NPM-ALK promotes GzB expression in ALK+ ALCL. A. Western blots (left) and quantification (right) of GzB and JunB expression in lysates from Karpas 299 or SUP-M2 cells transfected with 100 nM of the indicated pooled siRNAs. Note: the anti-NPM blots demonstrate NPM-ALK silencing. Molecular mass markers are indicated to the left of the blots. B. qRT-PCR analysis of GzB mRNA expression in Karpas 299 and SUP-M2 cells transfected with the indicated siRNAs. Note: these experiments were performed on the mRNA extracted from cells transfected in A. C. GzB promoter luciferase activity was measured in Karpas 299 cells transfected with 100nM of non-targeting (control) or ALK siRNA. Luciferase activity is expressed relative to the activity present in cells transfected with the pGL2-GzB promoter and control siRNA (which was set at 100%). In all experiments, the quantification represents the mean and standard deviation of three independent experiments. p values were obtained using paired, one-tailed t-tests.