Figure 5.

NPM-ALK regulates Perforin expression in ALK+ ALCL. A, Western blots (left) and quantification (right) of Perforin expression in lysates from Karpas 299 or SUP-M2 cells transfected with 100 nM of non-targeting (control) or ALK siRNA. B, qRT-PCR analysis of Perforin mRNA expression in Karpas 299 and SUP-M2 cells transfected with 100 nM of non-targeting (control) or ALK siRNA. Note: these experiments were performed on the mRNA extracted from cells transfected in A. C, Western blots (left) and quantification (right) of the indicated proteins in lysates from SU-DHL -1 cells transfected with 100 nM of non-targeting (control) or ALK siRNA. D, qRT-PCR analysis of Perforin and GzB mRNA expression in SU-DHL-1 cells transfected with 100 nM of non-targeting (control) or ALK siRNA. Note: these experiments were performed on the mRNA extracted from cells transfected in C. Molecular mass markers are indicated to the left of the western blots. In all experiments, the quantification represents the mean and standard deviation of three independent experiments. p values were obtained using paired, one-tailed t-tests. Note: the anti-NPM blots demonstrate NPM-ALK silencing.