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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Nanomedicine (Lond). 2011 Jan;6(1):25–42. doi: 10.2217/nnm.10.129

Figure 9.

Figure 9

Effect of catalase nanozymes on endosomal acidification in BMM. Mature mouse BMM were loaded with RITC-labeled catalase alone (A) or nanozymes (1 mg/ml, Z = 5); PEI50-PEG/catalase (B); PEI50/catalase (C), PGLU50-PEG/catalase (D), EPL/catalase (E), or PL10-PEG/catalase (F) for two hours, washed with PBS, and stained with 1 μM LysoSensor dye. Confocal images indicate that nanozymes comprising of catalase and positively-charged polymers, especially, with PEI block, substantially increased the pH in the compartments containing catalase. Oppositely, loading of BMM with negatively-charged containing nanozyme resulted in further decreases in endosomal pH compared to “naked” catalase.