. Author manuscript; available in PMC: 2012 Jan 1.

Published in final edited form as: Nanomedicine (Lond). 2011 Jan;6(1):25–42. doi: 10.2217/nnm.10.129

Table 5.

Preservation of catalase enzymatic activity in BIC by EPR^a, ^b

Formulation	Z ratio	Incubation time
Formulation	Z ratio	50 min	100 min	150 min
Catalase alone^c	0	35.1 ± 2	6.8 ± 1	3.1 ± 0.1

PEI₅₀-PEG/catalase	1	47.4 ± 1 (ns)	33.7 ± 1 (**)	15.8 ± 3 (**)
	10	67.5 ± 4 (**)	78.9 ± 8 (**)	77.2 ± 6 (**)
	20	68.9 ± 3 (**)	88.4 ± 3 (**)	81.4 ± 4 (**)

PL₁₀-PEG/catalase	1	68.2 ± 4 (*)	61.4 ± 3 (**)	57.7 ± 4 (**)
	10	88.2 ± 2 (**)	89.9 ± 3 (**)	79.0 ± 3 (**)
	20	93.9 ± 6 (**)	97.4 ± 8 (**)	91.5 ± 5 (**)

PGLU₅₀-PEG/catalase	1	49.5 ± 2 (ns)	46.0 ± 3 (**)	57.9 ± 4 (**)
	10	85.8 ± 4 (**)	82.1± 3 (**)	78.4 ± 3 (**)
	20	97.8 ± 5 (**)	91.2 ± 8 (**)	80.1 ± 3 (**)

Activity of each nanozyme is expressed as a % of residual catalase activity vs. initial activity at 0 time point

Statistical significance is shown by asterisk: p<0.05 (*), and p<0.005 (**) compared with residual activity of catalase alone at the same time point.

Catalase concentration was 250 ng/mL