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NO and expression of S239D-VASP enhance invasion through a collagen gel barrier. When indicated, SMCs were pretreated with detaNONOate (100 μM) for 30 minutes at room temperature. Invasion of SMCs through a thick collagen gel with PDGF-BB (10ng/mL) in the bottom chambers was investigated in the absence or presence of NO. Data (mean±SEM; n=3 experiments) are expressed as cells / mm2. * P ≤ 0.05.
