Table I.

Summary of Ca²⁺ Response Parameters with GCaMP2^a

	Condition	Response (% of Cells)	Ca2+ Waves (% of Cells)	Waves from PRTS (% of Waves)	Oscillationsb	wave velocity (μm/sec)	Laq Time (sec)
Ag	1700ng/ml	96.3	90	65	4.5 ± 0.3	42.6 ± 4	19.4 ± 1.25
	170ng/ml	94	87	54	4.9 ± 0.4	37.3 ± 5.1	19.5 ± 2.1
	17ng/ml	90.5	81	62	5 ± 0.4	18.7 ± 2.2	41.8 ± 4.4
	1.7ng/ml	53.8	78	86	3.7 ± 0.9	15.9 ± 1.6	42.6 ± 6.5
	0[Ca2+}o	96.8	89	31	1.5 ± 0.1	25.8 ± 4.5	30.2 ± 4.2
	50mM KCI	91	73	27.2	5.7 ± 0.5	27.8 ± 4.2	30.2 ± 4.9
	2mM Ba2+	64	78	72	1.6 ± 0.3	46.9 ± 3.5	20.9 ± 0.8
	2mM Sr2+	90	89	75	1.7 ± 0.2	44.4 ± 3.6	19.4 ± 1.5
	TG	76.2	43.7	71	1.7 ± 0.2	30.3 ± 16.4	29.1 ± 4
	A23187	73.9	44.8	30.7	2 ± 0.2	19.9 ± 5.3	17.9 ± 2.7
Ag	U73122 1μM	100	72	35	4 ± 0.5	47.2 ± 11	14.8 ± 2.3
	U73122 2μM	100	58	33	4.2 ± 0.4	47.2 ± 7.5	16.9 ± 2.3
	U73122 2.5μM	30	13	-	1 ± 0.2
	U73122 3.3μM	25	16	-	1 ± 0.3
	U73122 5μM	0	-	-	-
	2-APB	95.8	86	84	1.6 ± 0.2	25.9 ± 4	27.5 ± 2.7
	sphingosine	26.9	15	75	4.4 ± 0.8	27.8 ± 4.7	49.8 ± 5.7
	DM-sphingosine	62.1	15	33	2.8 ± 0.5	41.2 ± 9	29.3 ± 6.3
	Nifedipine	91	93	43	4.6 ± 0.3	26.8 ± 3.8	12.8 ± 0.8
	1μM Gd3+	80	87	69	3.7 ± 0.7	40.1 ± 6.2	22.7 ± 2.9
	10μM Gd3+	80.8	89	50	1.8 ± 0.1	21.4 ± 3	20.8 ± 2.3

^aUnless otherwise stated Ag was used at 1700ng/ml; number of cells in each experiment was 16-80 as specified in text or figure legends

^bDuring first two minutes of response

^*Data are mean ± SEM