Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Jan 24;6:10. doi: 10.1186/1750-1326-6-10

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright ©2011 Davis et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Three-dimensional reconstruction of cofilin-stained rods in deconvolved confocal image stacks from organotypic slices. Treatment of organotypic slices with Aβd/t results in a profound increase in cofilin-immunostained rods in the dentate gyrus/mossy fiber tract (DG/MFT) and a global change in cofilin distribution in cells in this region. (A) Single focal plane of non-rod-forming region near the CA3 compared to a rod hot spot in the dentate gyrus. Rods are evident in this single plane. (B) Three dimensional stack of planes from a cofilin stained control and Aβd/t-treated slice. Deconvolution of the confocal image stacks and thresholding the image by removing the lowest 20% of signal (lower panels) provides striking evidence of rod formation in this region. Hundreds of rods can be observed, which contain virtually all of the remaining immunostained cofilin.