Figure 11. The DRs in PnapA are required for BosR binding.

(A) Detailed sequences of the probes used in EMSAs. PnapA1 represents the BosR-protected region identified by Boylan, et al. [29]. PnapA2 represents the probe used by Katona, et al. [28]. The DRs are underlined. Mutated nucleotides are indicated in boldface. (B) EMSAs. The concentration of BosR (nM) is designated above each lane. Probe name is indicated below the image. Bound DNA is denoted by arrows. The numbers in parenthesis indicate numbers of omitted nucleotides (dash lines) between the probe and the ATG start codon.