Figure 4. Signaling pathways regulating FAK activation downstream of G_12/13 pathways.

Aspirin-treated, washed human platelets were pre-treated with different inhibitors (as indicated) for 5 minutes at 37°C followed by stimulation with AYPGKF (500 µM) for 90 seconds under stirring conditions at 37°C in an aggregometer. The lysates were then subjected to western blotting analysis and probed with anti- phospho FAK (Y-397) and total FAK antibodies as lane loading control (A &C) or anti- phospho- Src (416) and total c-Src antibodies as lane loading control (E). Quantitative data, normalized to the lane loading control, are representative of mean ± S.E.M (n = 3). The data was analyzed by ANOVA and * P≤0.05 was considered significant (B, D, and F).