Figure 4. Coverage of the 3D epistasis map.

The total number of antagonistic and synergistic interactions for each phenotype are displayed as stacked bars, with the cumulative sum of interactions at a given point in the list being represented by a black circle. Phenotypes were sorted according to the number of unique interactions added to a cumulative tally. It should be noted that the first phenotype in the list is the biomass flux, which does not have the maximal number of interactions. It was placed first so as to clearly demonstrate the gain in interaction coverage by observing non-growth phenotypes. Only the first 83 phenotypes are shown, as the number of unique interactions reaches saturation at this point.