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. 2011 Feb 10;7(2):e1001297. doi: 10.1371/journal.pgen.1001297

Figure 3. lpr1 and lpr2 are required for the uptake of neutral lipids by wing imaginal disc cells.

Figure 3

(A–D) Wild-type (A), Df(3R) lpr1/2 (B), Df(3R)lpr1 (C) and Df(3R)lpr2 (D) wing imaginal discs stained with nile red to reveal lipid droplets (yellow). In lpr1-, lpr2− double mutant there is a sharp decrease in lipid accumulation (B) whereas lpr2− discs display a mild reduction (D). (E, F) in situ hybridization to detect lpr2 (E) and lpr1 (F) mRNA expression in wild-type wing imaginal discs. Both genes are mainly expressed in the wing pouch area. Insets show a Df(3R)lpr1/2 disc (E) and a Df(3R)lpr1 disc (F) as negative controls. (G–H) Immunostaining with α-Lpr2 antibody detected expression in the wing pouch area of wild-type imaginal discs with minimal expression near the antero-posterior (arrow) and dorso-ventral compartment boundaries. (H) Magnification of the disc shown in (G). Lpr2 accumulates at baso-lateral membranes. Scale bars: 100 µm (A, G) and 25 µm (H). (A–F) shown at the same magnification.