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. 2011 Feb 10;7(2):e1001297. doi: 10.1371/journal.pgen.1001297

Figure 7. Lpr2E stabilizes lipophorins in the extracellular space.

Figure 7

(A–D) Df(3R)lpr1/2 wing imaginal discs overexpressing the lipid uptake-promoting UAS-lpr2E isoform (A–B) or the lipid uptake-defective UAS-lpr2F isoform (C–D) in the posterior compartment driven by en-gal4, shown in green. A modified immunostaining protocol was used to detect extracellular lipophorin (red, also shown in a separate panel in grey). (A, C) Optical cross-sections. Apical (a) and basal (b) sides as indicated. (B, D) Optical basal section. Cells overexpressing Lpr2E displayed increased accumulation of extracellular lipophorins at basolateral membranes near the basal side of the imaginal disc (arrows in A and B). The accumulation was more prominent near the antero-posterior compartment boundary, a region that also displayed higher Lpr2E protein levels. No noticeable changes in extracellular lipophorin were detected in cells overexpressing UAS-lpr2F (C–D). Scale bar: 10 µm. All panels are shown at the same magnification.