Fig. 4.

Colocalization of Cl^– and K⁺ channels in excised patches. a Current–voltage relationship of single-channel currents recorded an outside-out patch in the presence of 15 mM Cl^– in the medium at a PCO₂ of 70 mmHg. The holding potential was changed from +40 to –70 mV in 10-mV steps (left to right). The inset shows the summary graph comparing the I–V relations for control (n = 7, black squares) and lowered Cl^– (open circles, n = 3). Bars are SEMs. b A continuous current record from the patch before and during the application of low Cl^– aCSF. Portions of the record in control aCSF (i) and low Cl^– aCSF (ii) are shown below. Note that the reduction of extracellular Cl^– ions reduces both the holding current and basal noise. Outside-out patch held at +10 mV