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. 2010 Sep-Oct;1(5):422–431. doi: 10.4161/nucl.1.5.12903

Figure 1.

Figure 1

NLS-IN and SV40-NLS peptides disrupt the IN-importin α but not IN-TNPO3 complex in virus infected cells. SupT1 lymphocytes were infected with wild-type HIV-1 and incubated in the presence or absence of the indicated peptides. Following infection, cells were lysed and half of the cells' lysate volume was subjected to SDS-PAGE, then immunoblotted with either anti-importin α (anti-Impα), anti-TNPO3, anti-IN or anti-Rev antibodies. The complementary HRP-conjugated antibodies were used as the second antibody. The remaining lysate was subjected to co-IP with either the anti-Impα, anti-TNPO3, anti-IN or anti-Rev antibodies. Following SDS-PAGE, the gels were immunoblotted by each of the above antibodies and then incubated with the complementary HRP-conjugated antibodies as second antibody. (A) No peptide; (B) SV40-NLS-Pen peptide; (C) NLS-IN-Pen peptide. When peptides were used, cells were incubated with 150 µM of the indicated peptide.