Figure 2.

Disruption of IN-TNPO3 but not IN-importin α complex by the Rev 53–67 and IN-1 peptides. SupT1 lymphocytes were infected with wild-type HIV-1, incubated with the indicated peptides and subjected to co-IP and immunoblotting as described in the legend to Figure 1 and Methods. (A) IN-1 peptide; (B) Rev 53–67 peptide; (C) Rev ARM peptide; (D) similar to (B) and (C) but with the anti-Rev and anti-importin β (anti-Impβ) antibodies and with IN-expressing SupT1 cells in the right lane. When peptides were used, cells were incubated with 150 µM of the indicated peptide. Other experimental details are described in Methods.