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. Author manuscript; available in PMC: 2011 Feb 11.
Published in final edited form as: Mol Genet Genomics. 2008 Aug 21;280(5):385–396. doi: 10.1007/s00438-008-0372-z

Figure 1.

Figure 1

The lawc gene corresponds to CG32711. (A) Wing and arista phenotypes of ctn lawcP1 (mutant) and ctn lawcP1; P[CG32711 rescue construct] (rescue) flies are shown with statistical significance represented by α values indicated above each rescue bar. (B) Transcript levels obtained by qRT-PCR from three separate larval extracts of each genotype (hemizygous ctn (+) and hemizygous ctn lawcP1 (P1)) were normalized to Act5C. One + extract was set as the reference and ΔΔCt values for each extract were calculated and then averaged. Graphs report results with C32711, Trf2, and CG11190 primers. (C) Western analysis of ctn (+) and ctn lawcP1 (P1) third instar larvae using α-Lawc antibodies confirms the reduction in CG32711/lawc gene expression detected by qRT-PCR. α-Lamin was used as a loading control. (D) Predicted amino acid sequence of the Lawc protein.