Lawc is recruited to ecdysone puffs after transcription has begun. (A, B, and C) Polytene chromosomes from wild-type third instar larvae were incubated with α-Lawc and (A) α-Pol IIoSer2 (H5) (green), (B) H14 (green), or (C) α-EcR (red). The PS of each larva was determined as described in the text. The white arrows point to 74EF, the blue arrows indicate 75B and the yellow arrows point to 78D.