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. Author manuscript; available in PMC: 2011 Feb 11.
Published in final edited form as: Mol Genet Genomics. 2008 Aug 21;280(5):385–396. doi: 10.1007/s00438-008-0372-z

Figure 4.

Figure 4

Lawc is recruited to heat-shock puffs after transcription has begun. Images are shown with 87A on the left (white arrow) and 87C on the right (blue arrow). (A) Combined FISH-immunofluorescence was performed on wild-type polytene chromosomes with DAPI (blue), an Hsp70 probe (red), and α-Lawc. (B) Wild-type larvae were placed at 37° (HS) for the indicated amount of time. Polytene chromosomes were incubated with DAPI (blue), α-Lawc, and α-Pol IIo (green). (C) The experiment was carried out as in (A) except larvae were exposed to 37° and then allowed to recover at 25°.