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. Author manuscript; available in PMC: 2011 Feb 11.
Published in final edited form as: Mol Genet Genomics. 2008 Aug 21;280(5):385–396. doi: 10.1007/s00438-008-0372-z

Figure 5.

Figure 5

The amount of Pol IIoSer2 is augmented in the lawcP1 mutant but transcript levels are not. (A) Western analysis on hemizygous ctn (+) and hemizygous ctn lawcP1 (P1) third instar larvae with H14 and H5 antibodies shows an increase of the Pol IIoSer2 form in the ctn lawcP1 mutants. α-Lamin was used as a loading control. (B and C) Combined FISH-immunofluorescence with α-Lawc was performed on polytene chromosomes from wild-type male third instar larvae (wt) or ctn third instar male larvae. FISH probes (green) were made to either (B) CG9497 or (C) ACXD. Graphs depict the result of qRT-PCR experiments comparing (B) CG9497 and (C) ACXD transcript levels in hemizygous ctn (+) and hemizygous ctn lawcP1 (P1) third instar larval salivary glands. Relative transcript levels are calculated as described for Figure 1.