Skip to main content
. 2011 Feb 16;100(4):1024–1033. doi: 10.1016/j.bpj.2011.01.007

Figure 2.

Figure 2

Lifetime images of a rigor myofibril. The color bar to the right of image A is the lifetime scale from 0 (blue) to 6 (red) ns. The red circle is a projection of the confocal aperture on the sample plane (diameter 1.2 μm). Native LC1 molecules were exchanged in myofibrils with 4 nM (A), 2 nM (B), 1.2 nM (C), and 0.8 nM (D) of R-LC1. Sarcomere length = 3.1 μm (A), 3.0 μm (B), 3.0 μm (C), and 2.9 μm (D). Images were acquired on a PicoQuant Micro Time 200 confocal lifetime microscope. The sample was excited with a 470 nm pulse of light and the emission was collected on an APD through a LP500 filter.