Figure 5.

Examples of histograms of polarized fluorescence from rigor (top panel) and relaxed (bottom panel) myofibrils. Horizontal scale: The negative value of parallel fluorescence polarization. Vertical scale: The number of events during 20 s. The position of peaks for rigor myofibrils were (top panel, clockwise from the top left): P = −0.07, P = 0.05, P = 0.08, P = 0.02, P = 0.00, P = 0.07. The blue line shows the fit to a single Gaussian distribution. For relaxed myofibrils (bottom panel), the parameters (area, center, width, and height) for each peak of a two Gaussian fit are given in clockwise order, starting from the upper left. Upper left: minor peak (red) 0.85, 0.18, 0.10, 6.82; major peak (green) 14.89, −0.03, 0.21, 57.01. Upper middle: minor peak (red) 0.64, 0.03, 0.00, 115.88; major peak (green) 17.16, −0.02, 0.22, 61.66. Upper right: minor peak (red) 0.11, 0.11, 0, 86.96; major peak (green) 12.63, −0.07, 0.19, 51.74. Lower left: minor peak (red) 1.09, 0.05, 0.09, 10.09; major peak (green) 12.10, 0.09, 0.18, 53.36. Lower middle: minor peak (red) 0.23, 0.05, 0.02, 9.54; major peak (green) 14.24, −0.07, 0.21, 53.62. Lower right: minor peak (red) 0.47, 0.01, 0.05, 7.57; major peak (green) 14.30, −0.00, 0.23, 48.84. The contribution of the minor peak (red line) makes little difference relative to the second peak (green line) of a double Gaussian distribution fit. The blue line is the sum of red and green line fits.