Figure 6.

Examples of histograms of polarized fluorescence from contracting myofibrils. Horizontal scale: The negative value of fluorescence polarization. Vertical scale: The number of events during 20 s. The positions of minor (red line) and major (green line) peaks from a double Gaussian distribution fit are (clockwise from the top left) P = −0.3 and 0.11, P = −0.29 and −0.06, P = −0.2 and −0.06, P = −0.33 and −0.14, P = −0.32 and −0.11, and P = −0.31 and .012. The ratios of the major peak (green line) to the corresponding minor peak (red line) in the double Gaussian fits are (clockwise, from the top left corner) 2.7, 1.1, 0.5, 2.7, 1.6, and 3.1. The blue line is the sum of red and green line fits.