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Standard fermentation for a S. flexneri 2a culture harboring plasmid pGLNic: (a) IPTG-addition at −45 min to induce biosynthesis of SNUC, (b) temperature up-shift to 42°C at 0 min to induce lysis, (c) pH up-shift to 8.0 and addition of Mg2+ and Ca2+ at +90 min to activate the enzymatic function of SNUC.
