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The pHg/pSILBAγ vector system. The ihpRNA expression cassette is constructed in pSILBAγ under A. bisporus gpdII promoter by two directed cloning steps. Laccaria Lbnr intron forms the spacer for the inverted repeats. The hygromycin B resistance cassette is located in the T-DNA of the binary vector pHg. Joining of pSILBAγ and pHg creates the AMT/silencing/rescue-vector with a predicted T-DNA structure.
