FIGURE 2.

TGFBIp contains a trypsin-resistant core. Limited proteolysis of TGFBIp by trypsin. A, SDS-PAGE of trypsin digests. Trypsin to TGFBIp ratios are indicated at the top. B, LC-MS/MS analyses of analyzed fragments. C, schematic representation showing approximate trypsin cleavage sites in TGFBIp (arrows) based on peptide mass fingerprinting. The scissors indicate the C-terminal truncation reported in TGFBIp purified from human corneas (16). A large part of TGFBIp is resistant to trypsin proteolysis.