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Far-UV CD spectroscopy of purified FAS1-4 variants. Far-UV circular dichroism spectra of the FAS1-4 variants WT, A546T, R555Q, and R555W. All FAS1-4 variants have similar CD spectra indicating that their ratios of secondary structure elements are identical. The minimum at ∼210 nm together with the slight dip at ∼222 nm are characteristic of α+β structures.
